DNA Content and Chromosome Complement ofChromis Chromis(Pommacentridae, Perciformes)

Location of ribosomal DNA in the human chromosome complement.

Hybridization of (3)H-labeled ribosomal RNA to human chromosomes on slides resulted in specific labeling of the satellite regions of chromosomes 13, 14, 15, 21, and 22, with an over-all efficiency of about 5%. Differences between D and G chromosomes, and between associated and unassociated satellites, were not significant. Labeling of all other parts of the preparations was nonspecific, and inc...

X-Chromosome Complement and Estrogen

DNA barcoding of gobiid fishes (Perciformes, Gobioidei).

Gobiids constitute a major proportion of fish population in both tropical and temperate freshwater as well as marine ecosystem. Due to their small size, cryptic ecology and ambiguous morphological characters, gobiids diversity was not documented completely. In this study, DNA barcodes were generated for 11 species of gobiids, collected from the Ashtamudi Lake, India. The mitochondrial COI gene ...

DHPLC Applications: Finding DNA Variation on the Y Chromosome

Denaturing High-Performance Liquid Chromatography (DHPLC) is a recently developed technique forthe detection of single nucleotide polymorphisms (SNPs) and mutations. It involves the comparisonbetween two or more DNAs as a mixture of denatured and reannealed PCR products. The methodologyis based on the principle of reversed phase liquid chromatography and uses a unique DNA sepa...

Cyclosporine Effects on Chick Limb Bud Mesenchyme and DNA Content

Background: Cyclosporine A (CsA) is a powerful immunosuppressive agent, which is used for the prevention of allograft rejection and for the treatment of autoimmune diseases.  Many transplant recipients must take this medicine for the rest of their lives.  Females in reproductive age group on prolonged CsA therapy have legitimate concerns about drug effects on pregnancy. Objective: To explore Cs...